Rationale for the real-time and dynamic cell death assays using propidium iodide.

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Title

Rationale for the real-time and dynamic cell death assays using propidium iodide.

Content partner

The University of Auckland Library

Collection

ResearchSpace@Auckland

Description

We have recently reported an innovative approach to use charged fluorochromes such as propidium iodide (PI) in the real-time, dynamic cell viability assays. This study was designed to provide a mechanistic rationale for the kinetic assays using cell permeability markers. Uptake of PI by live cells, effect on the cell cycle, long-term proliferation capacity, DNA damage response, and pharmacologic interactions with anticancer drugs were studied using both laser scanning microscopy and laser sca...

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Format

Research paper

Research format

Journal article

Date created

2010-04

Creator

Zhao, H / Oczos, J / Janowski, P / Trembecka, D / Dobrucki, J / Darzynkiewicz, Z / Wlodkowic, Donald

URL

http://hdl.handle.net/2292/9682

Related subjects

Biological Assay / Cell Death / Cell Line, Tumor / Cell Proliferation / DNA Replication / Fluorescence / Histones / Humans / Microscopy, Confocal / Phosphorylation / Propidium / Time Factors

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