MALDI imaging mass spectrometry of integral membrane proteins from ocular lens and retinal tissue.

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Title

MALDI imaging mass spectrometry of integral membrane proteins from ocular lens and retinal tissue.

Content partner

The University of Auckland Library

Collection

ResearchSpace@Auckland

Description

A tissue preparation protocol for MALDI (matrix-assisted laser desorption/ionization) imaging mass spectrometry of integral membrane proteins was developed using ocular lens and retinal tissues as model samples. Frozen bovine and human lenses were cryosectioned equatorially or axially at -20 degrees C into 20 mum-thick tissue sections. Lens sections were mounted onto gold-coated MALDI targets by methanol soft-landing to maintain tissue integrity. Tissue sections underwent extensive water wash...

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Format

Research paper

Research format

Journal article

Date created

2009

Creator

Grey, Angus / Chaurand, P / Caprioli, RM / Schey, KL

URL

http://hdl.handle.net/2292/17584

Related subjects

Acetonitriles / Animals / Aquaporins / Cattle / Child / Coumaric Acids / Eye Proteins / Gold / Humans / Lens, Crystalline / Membrane Proteins / Rabbits / Retina / Solvents / Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Water

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