A rapid and sensitive method to detect siRNA-mediated mRNA cleavage in vivo using 5' RACE and a molecular beacon probe

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Title

A rapid and sensitive method to detect siRNA-mediated mRNA cleavage in vivo using 5' RACE and a molecular beacon probe

Content partner

The University of Auckland Library

Collection

ResearchSpace@Auckland

Description

Specific detection of mRNA cleavage by 5'RACE is the only method to confirm the knockdown of mRNA by RNA interference, but is rarely reported for in vivo studies. We have combined 5'-RNA-linker-mediated RACE (5'-RLM-RACE) with real-time PCR using a molecular beacon to develop a rapid and specific method termed MBRACE, which we have used to detect small-interfering RNA (siRNA)-induced cleavage of ApoB, RRM1 and YBX1 transcripts in vitro, and ApoB in vivo. When RNA from siRNA-transfected cells ...

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Format

Research paper

Research format

Journal article

Date created

2010

Creator

Lasham, Annette / Herbert, M / 't Wallant, NC / Patel, R / Feng, S / Eszes, M / Cao, H / Reid, G

URL

http://hdl.handle.net/2292/17830

Related subjects

Science & Technology / Life Sciences & Biomedicine / Biochemistry & Molecular Biology / SMALL INTERFERING RNA / NONHUMAN-PRIMATES / INNATE IMMUNITY / DELIVERY / GENE / MICE / HYBRIDIZATION / AMPLIFICATION / PROSPECTS / INJECTION

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