Production of specific polyclonal antibodies anti-human factor IX

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Title

Production of specific polyclonal antibodies anti-human factor IX

Content partner

Directory of Open Access Journals

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Directory of Open Access Journals

Description

In this work, polyclonal antibodies anti-human Factor IX were produced in New Zealand rabbits by immunization with commercial pure human FIX (hFIX) (Octanyne®, Octapharma, USA). The serum containing immunoglobulins anti-hFIX was useful to detect hFIX antigen in human plasma fractions submitted to anionic exchange chromatographic process and with a large yield. Immunoassays (ELISA) using bovine serum albumin, trypsin and peptides generated by cleavage assays with trypsin as digestion enzyme wa...

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Format

Research paper

Date created

2006-07-01

Creator

Daniel Gonçalves Chaves / Cibele Velloso Rodrigues / Wanderley Almeida Ferreira / Pollyana Fantini Miranda Siqueira / Clara Guerra Duarte / Anna Bárbara de Freitas Carneiro-Proietti / Marcelo Matos Santoro

Contributing partner

Brazilian Archives of Biology and Technology

URL

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500010

Related subjects

Factor IX / hemophilia B / HPLC / purification / antibodies / ELISA / Biotechnology

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