Research paper

Testing neocortical slice viability in non-perfused no-magnesium artificial cerebrospinal fluid solutions

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Title
Testing neocortical slice viability in non-perfused no-magnesium artificial cerebrospinal fluid solutions
Content partner
The University of Auckland Library
Collection
ResearchSpace@Auckland
Description

The acute in vitro brain slice model is a widely used neurophysiological research tool. When applying this method, most researchers continuously perfuse slices with carbogenated artificial cerebrospinal fluid (ACSF) to maintain pH balance and tissue oxygen delivery. Common wisdom suggests that static recordings are incompatible with submerged bath methodology because of deficiency in tissue oxygen supply. However, to our knowledge this has not been tested. In this study, we wanted to determin...

Format
Research paper
Research format
Journal article
Date created
2012-03-15
Creator
Voss, LJ / George, SA / Sleigh, James
URL
http://hdl.handle.net/2292/32041
Related subjects
Action Potentials / Animals / Carbon Dioxide / Cerebrospinal Fluid / Female / Hydrogen-Ion Concentration / Magnesium / Male / Mice / Mice, Inbred C57BL / Neocortex / Oxygen / Perfusion / Seizures / Time Factors / Tissue Culture Techniques

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