Preparation of polyclonal antibody of sodium dependent neutral amino acid transporter-2 (SNAT2)

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Title

Preparation of polyclonal antibody of sodium dependent neutral amino acid transporter-2 (SNAT2)

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Directory of Open Access Journals

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Directory of Open Access Journals

Description

Sodium-dependent neutral amino acid transporter 2 (SNAT2),transport of small neutral amino acids,is the second member of the SLC38 family and widely expresses in mammals.SNAT2 dysfunction can lead to many neurological diseases,such as Alzheimer disease and Parkinson's disease.In order to prepare SNAT2 special antibody,the gene of 75 amino acids of the N terminal of SNAT2 was amplied by PCR from pBK-CMVΔ-SNAT2-HA plasmid.The expression plasmid pET28a-SNAT2-75aa was constructed and transformed ...

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Format

Research paper

Date created

2013-10-01

Creator

LIU Li / MIN Weiyong / ZHANG Zhou

Contributing partner

Journal of Shanghai Normal University (Natural Sciences)

URL

http://qktg.shnu.edu.cn/zrb/shsfqkszrb/ch/reader/create_pdf.aspx?file_no=201305007&year_id=2013&q...

Related subjects

transporter / neutral amino acid / antibody / protein purification / Science (General)

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