The Vital Dye CDr10b Labels the Zebrafish Mid-Intestine and Lumen.

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Title

The Vital Dye CDr10b Labels the Zebrafish Mid-Intestine and Lumen.

Content partner

The University of Auckland Library

Collection

ResearchSpace@Auckland

Description

We describe the use of the fluorescent reporter compound CDr10b to label mid-intestinal structures in zebrafish larvae after simple immersion. CDr10b is deposited into the gut where it initially fills the lumen and is excreted. Using laser-mediated injury of the intestine, we show that CDr10b provides a useful readout of the integrity and repair of the epithelial cell barrier. In addition, CDr10b specifically labels the absorptive mid-intestine segment that is analogous to the mammalian small...

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Format

Research paper

Research format

Journal article

Date created

2017-03-13

Creator

Sander, Veronika / Patke, Shantanu / Lee, Jung Y / Chang, Young-Tae / Davidson, Alan

URL

http://hdl.handle.net/2292/40510

Related subjects

Intestines / Animals / Zebrafish / Boron Compounds / Tretinoin / Microscopy, Fluorescence / Signal Transduction / Larva

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