Evolution and Evaluation of Engineered DNA Ligases for Improved Blunt-End Ligation

About this item

Title

Evolution and Evaluation of Engineered DNA Ligases for Improved Blunt-End Ligation

Content partner

Victoria University of Wellington

Collection

Open Access Victoria University of Wellington

Description

DNA ligases are fundamental enzymes in molecular biology and biotechnology where they perform essential reactions, e.g. to create recombinant DNA and for adaptor attachment in next-generation sequencing. T4 DNA ligase is the most widely used commercial ligase owing to its ability to catalyse ligation of blunt-ended DNA termini. However, even for T4 DNA ligase, blunt-end ligation is an inefficient activity compared to cohesive-end ligation, or its evolved activity of sealing single-strand nick...

Read more

Format

Research paper

Research format

Scholarly text / Thesis

Thesis level

Masters

Date created

2023

Creator

Sharma, Janine

URL

https://figshare.com/articles/thesis/Evolution_and_Evaluation_of_Engineered_DNA_Ligases_for_Impro...

Related subjects

Analytical biochemistry / Enzymes / DNA ligase / Directed Evolution / Competitive Ligase Selection / Protein Purification / Gel-Based Assay / Ligation / School: School of Biological Sciences / Unit: Centre for Biodiscovery / Analytical Biochemistry / Degree Discipline: Cell and Molecular Bioscience / Degree Level: Masters / Degree Name: Master of Science

What is the copyright status of this item?

Share

See below for specifics about how you may use this item.

More Information

Victoria University of Wellington has this to say about the rights status of this item:

CC BY-NC-ND 4.0

What can I do with this item?

You must always check with Victoria University of Wellington to confirm the specific terms of use, but this is our understanding:

Non-infringing use

NZ Copyright law does not prevent every use of a copyright work. You should consider what you can and cannot do with a copyright work.

Share it

This item is suitable for copying and sharing with others, without further permission.

No modifying

You are not allowed to adapt or remix this item into any other works.

No commercial use

You may not use this item commercially.