Quantitative high-throughput assay to measure MC4R-induced intracellular calcium.

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Title

Quantitative high-throughput assay to measure MC4R-induced intracellular calcium.

Content partner

The University of Auckland Library

Collection

ResearchSpace@Auckland

Description

The melanocortin-4 receptor (MC4R), a critical G-protein-coupled receptor (GPCR) regulating energy homeostasis, activates multiple signalling pathways, including mobilisation of intracellular calcium ([Ca2+]i). However, very little is known about the physiological significance of MC4R-induced [Ca2+]i since few studies measure MC4R-induced [Ca2+]i. High-throughput, read-out assays for [Ca2+]i have proven unreliable for overexpressed GPCRs like MC4R, which exhibit low sensitivity mobilising [Ca...

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Format

Research paper

Research format

Journal article

Date created

2021-4-26

Creator

Kumar, Shree Senthil / Ward, Marie-Louise / Mountjoy, Kathleen Grace

URL

https://hdl.handle.net/2292/58370

Related subjects

Humans / Calcium / Receptor, Melanocortin, Type 4 / Cyclic AMP / Signal Transduction / Calcium Signaling / Amino Acid Sequence / Protein Binding / Energy Metabolism / Homeostasis / High-Throughput Screening Assays / HEK293 Cells / G-protein-coupled receptor / calcium / cell signalling / high-throughput screening / melanocortin-4 receptor / Science & Technology / Life Sciences & Biomedicine / Endocrinology & Metabolism / MITOGEN-ACTIVATED PROTEIN / ENDOTHELIAL-CELLS / IN-VITRO / FURA-2 / CA2+ / SEQUESTRATION / MOBILIZATION / PHARMACOLOGY / INHIBITION / MUTATIONS / 0707 Veterinary Sciences / 1103 Clinical Sciences / 1114 Paediatrics and Reproductive Medicine

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