Phospholipid fatty acid (PLFA) analysis as a tool to estimate absolute abundances from compositional 16S rRNA bacterial metabarcoding data.

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Title

Phospholipid fatty acid (PLFA) analysis as a tool to estimate absolute abundances from compositional 16S rRNA bacterial metabarcoding data.

Content partner

The University of Auckland Library

Collection

ResearchSpace@Auckland

Description

Microbial biodiversity monitoring through the analysis of DNA extracted from environmental samples is increasingly popular because it is perceived as being rapid, cost-effective, and flexible concerning the sample types studied. DNA can be extracted from diverse media before high-throughput sequencing of the prokaryotic 16S rRNA gene is used to characterize the taxonomic diversity and composition of the sample (known as metabarcoding). While sources of bias in metabarcoding methodologies are ...

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Format

Research paper

Research format

Journal article

Date created

2021-09

Creator

Lewe, Natascha / Hermans, Syrie / Lear, Gavin / Kelly, Laura T / Thomson-Laing, Georgia / Weisbrod, Barbara / Wood, Susanna A / Keyzers, Robert A / Deslippe, Julie R

URL

https://hdl.handle.net/2292/59360

Related subjects

Bacteria / Fatty Acids / Phospholipids / RNA, Ribosomal, 16S / Soil / Soil Microbiology / Biodiversity / Biomass / Fresh Water / Environmental Monitoring / Geologic Sediments / Cost-Benefit Analysis / High-Throughput Nucleotide Sequencing / Biomarker / Environmental substrates / Microbial biomass / PLFA / eDNA / Genetics / Science & Technology / Life Sciences & Biomedicine / Biochemical Research Methods / Microbiology / Biochemistry & Molecular Biology / Enviromental substrates / SOIL MICROBIAL COMMUNITIES / ENVIRONMENTAL DNA / FRESH-WATER / EXTRACTION / DIVERSITY / QUANTIFICATION / PROFILES / SEDIMENT / BIAS / 0605 Microbiology / 1108 Medical Microbiology

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