Mapping Glucose Uptake, Transport and Metabolism in the Bovine Lens Cortex.

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Title

Mapping Glucose Uptake, Transport and Metabolism in the Bovine Lens Cortex.

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The University of Auckland Library

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ResearchSpace@Auckland

Description

Purpose: To spatially correlate the pattern of glucose uptake to glucose transporter distributions in cultured lenses and map glucose metabolism in different lens regions. Methods: Ex vivo bovine lenses were incubated in artificial aqueous humour containing normoglycaemic stable isotopically-labelled (SIL) glucose (5 mM) for 5 min-20 h. Following incubations, lenses were frozen for subsequent matrix-assisted laser desorption/ionisation (MALDI) imaging mass spectrometry (IMS) analysis using hi...

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Format

Research paper

Research format

Journal article

Date created

2022-01

Creator

Zahraei, Ali / Guo, George / Varnava, Kyriakos G / Demarais, Nicholas J / Donaldson, Paul J / Grey, Angus C

URL

https://hdl.handle.net/2292/60483

Related subjects

MALDI imaging mass spectrometry (IMS) / glucose / glucose transporter / lens / metabolites / Eye Disease and Disorders of Vision / 1.1 Normal biological development and functioning / 1 Underpinning research / 0606 Physiology / 1116 Medical Physiology / 1701 Psychology

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