Validation of a molecular assay to detect SARS-CoV-2 in saliva

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Title

Validation of a molecular assay to detect SARS-CoV-2 in saliva

Content partner

Victoria University of Wellington

Collection

Open Access Victoria University of Wellington

Description

AIM: To validate a reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) assay to detect SARS-CoV-2 in saliva in two independent Aotearoa New Zealand laboratories. METHODS: An RT-qPCR assay developed at University of Illinois Urbana-Champaign, USA, was validated in two New Zealand laboratories. Analytical measures, such as limit of detection (LOD) and cross-reactivity, were performed. One hundred and forty-seven saliva samples, each paired with a contemporaneously collected n...

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Format

Research paper

Research format

Scholarly text / Journal article

Date created

2021

Creator

Janet Pitman / AJ Morris / S Grice / JT Walsh / L Wang / MD Burke / A Dixon-McIver

URL

https://figshare.com/articles/journal_contribution/Validation_of_a_molecular_assay_to_detect_SARS...

Related subjects

COVID-19 / COVID-19 Testing / Clinical Laboratory Techniques / Humans / New Zealand / SARS-CoV-2 / Saliva / Sensitivity and Specificity / Prevention / Clinical Research / .2 Evaluation of markers and technologies / Detection, screening and diagnosis / Medical and Health Sciences / General & Internal Medicine

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