Development of Multiwell-Plate Methods Using Pure Cultures of Methanogens To Identify New Inhibitors for Suppressing Ruminant Methane Emissions

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Title

Development of Multiwell-Plate Methods Using Pure Cultures of Methanogens To Identify New Inhibitors for Suppressing Ruminant Methane Emissions

Content partner

University of Otago

Collection

Otago University Research Archive

Description

Hydrogenotrophic methanogens typically require strictly anaerobic culturing conditions in glass tubes with overpressures of H and CO that are both time-consuming and costly. To increase the throughput for screening chemical compound libraries, 96-well microtiter plate methods for the growth of a marine (environmental) methanogen strain S2 and the rumen methanogen species AbM4 were developed. A number of key parameters (inoculum size, reducing agents for medium preparation, assay duration, inh...

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Format

Research paper

Research format

Scholarly text / Journal article

Thesis level

Article

Date created

2017-08-01

Creator

Weimar, M R / Cheung, J / Dey, D / McSweeney, C / Morrison, M / Kobayashi, Y / Whitman, W B / Carbone, V / Schofield, L R / Ronimus, R S / Cook, G M

URL

https://hdl.handle.net/10523/26305

Related subjects

Animals / Biological Products - pharmacology / Culture Techniques - instrumentation / Culture Techniques - methods / Drug Evaluation, Preclinical / Methane - metabolism / Methanobrevibacter - drug effects / Methanobrevibacter - growth & development / Methanobrevibacter - metabolism / Methanococcus - drug effects / Methanococcus - growth & development / Methanococcus - metabolism / Rumen - metabolism / Rumen - microbiology / Ruminants - metabolism / Ruminants - microbiology

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