A Comparison of Real-Time and Endpoint Cell Viability Assays for Improved Synthetic Lethal Drug Validation

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Title

A Comparison of Real-Time and Endpoint Cell Viability Assays for Improved Synthetic Lethal Drug Validation

Content partner

University of Otago

Collection

Otago University Research Archive

Description

Cell viability assays fulfill a central role in drug discovery studies. It is therefore important to understand the advantages and disadvantages of the wide variety of available assay methodologies. In this study, we compared the performance of three endpoint assays (resazurin reduction, CellTiter-Glo, and nuclei enumeration) and two real-time systems (IncuCyte and xCELLigence). Of the endpoint approaches, both the resazurin reduction and CellTiter-Glo assays showed higher cell viabilities wh...

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Format

Research paper

Research format

Scholarly text / Journal article

Thesis level

Article

Date created

2015-12-01

Creator

Single, Andrew / Beetham, Henry / Telford, Bryony J. / Guilford, Parry / Chen, Augustine

URL

https://hdl.handle.net/10523/30588

Related subjects

Biochemical Research Methods / Biochemistry & Molecular Biology / Biotechnology & Applied Microbiology / Chemistry / Chemistry, Analytical / Life Sciences & Biomedicine / Physical Sciences / Science & Technology

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