The CRISPR/Cas9 system and its applications in crop genome editing

About this item

Title

The CRISPR/Cas9 system and its applications in crop genome editing

Content partner

University of Otago

Collection

Otago University Research Archive

Description

The CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR associated protein9) system is an RNA-guided genome editing tool that consists of a Cas9 nuclease and a single-guide RNA (sgRNA). By base-pairing with a DNA target sequence, the sgRNA enables Cas9 to recognize and cut a specific target DNA sequence, generating double strand breaks (DSBs) that trigger cell repair mechanisms and mutations at or near the DSBs sites. Since its discovery, the CRISPR/Cas9 system has r...

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Format

Research paper

Research format

Scholarly text / Journal article

Thesis level

Article

Date created

2019-05

Creator

Bao, Aili / Burritt, David J / Chen, Haifeng / Zhou, Xinan / Cao, Dong / Tran, Lam-Son Phan

URL

https://hdl.handle.net/10523/36684

Related subjects

mutant screening / CRISPR/Cas9 ribonucleoproteins / Genome editing / CRISPR/Cas9 system / transgene-free / crop breeding

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