Isolation of Neutrophils from Larval Zebrafish and Their Transplantation into Recipient Larvae for Functional Studies

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Title

Isolation of Neutrophils from Larval Zebrafish and Their Transplantation into Recipient Larvae for Functional Studies

Content partner

University of Otago

Collection

Otago University Research Archive

Description

Live imaging of neutrophils within optically transparent larval zebrafish has proved a powerful technique to investigate how specific gene products control neutrophil function. To resolve whether a gene contributes to neutrophil function in a cell-autonomous manner necessitates a way to examine gene-deficient neutrophils in an otherwise wild type background. To this end, here we describe methods to harvest fluorescent neutrophils from larval donor zebrafish and transplant them into age-matche...

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Format

Research paper

Research format

Scholarly text / Journal article

Thesis level

Article

Date created

2020

Creator

Darroch, Hannah / Astin, Jonathan W / Hall, Christopher J

URL

https://hdl.handle.net/10523/39260

Related subjects

Animals / Cell Separation - methods / Cell Transplantation - methods / Fluorescent Antibody Technique / Genes, Reporter / Immunity, Innate / Larva / Molecular Imaging / Neutrophils - immunology / Neutrophils - metabolism / Phagocytosis / Zebrafish

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